zymoclean large fragment dna recovery kit (Zymo Research)
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zymoclean large fragment dna recovery kit
Zymoclean Large Fragment Dna Recovery Kit, supplied by Zymo Research, used in various techniques. Bioz Stars score: 95/100, based on 201 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/large+fragment+dna+recovery+kit/Zymoclean+Large+Fragment+DNA+Recovery/bio_rxiv__64898__2026__02__24__707731-185-22-28
Average 95 stars, based on 201 article reviews
Zymoclean Large Fragment Dna Recovery Kit, supplied by Zymo Research, used in various techniques. Bioz Stars score: 95/100, based on 201 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/large+fragment+dna+recovery+kit/Zymoclean+Large+Fragment+DNA+Recovery/bio_rxiv__64898__2026__02__24__707731-185-22-28
Average 95 stars, based on 201 article reviews
zymoclean large fragment dna recovery kit - by Bioz Stars,
2026-09
95/100 stars
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Purification:Article Title: Reference genome of the bicolored carpenter ant, Camponotus vicinus Article Snippet: DNA was further cleaned with Zymo gDNA clean and concentrator kit (Zymo Research, Irvine, CA; Cat # 4033). .. To retain large DNA fragments, columns from Article Title: Radical-Mediated Nucleophilic Peptide Cross-Linking in Dynobactin Biosynthesis Article Snippet: Reaction mix: 34.5 μ L water, 2.5 μ L DMSO, 10 μ L 5× Q5 Reaction Buffer, 200 μ M (final concentration) dNTPs, 0.5 μ M Dyn_Forward and Dyn_Reverse primers ( Table S1 ), 1 μ g template DNA, and 0.02 U/ μ L Q5 DNA polymerase (New England Biolabs, Ipswich, USA). .. PCR products were purified using an agarose gel electrophoresis and the Article Title: Radical-Mediated Nucleophilic Peptide Cross-Linking in Dynobactin Biosynthesis. Article Snippet: Dynobactins are recently discovered ribosomally synthesized and post-translationally modified peptide (RiPP) antibiotics that selectively kill Gram-negative pathogens by inhibiting the β-barrel assembly machinery (Bam) located on their outer membranes.. Such activity of dynobactins derives from their unique cross-links between Trp1-Asn4 and His6-Tyr8.. In particular, the His6-Tyr8 cross-link is formed between Nτ of His6 and Cβ of Tyr8, an unprecedented type of cross-link in RiPP natural products. Article Title: Reference genome of the bicolored carpenter ant, Camponotus vicinus. Article Snippet: DNA was further cleaned with Zymo gDNA clean and concentrator kit (Zymo Research, Irvine, CA; Cat # 4033). .. To retain large DNA fragments, columns from Article Title: Context-dependent neocentromere activity in synthetic yeast chromosome VIII Article Snippet: DNA sticky ends were filled with biotin-14-dCTP (Invitrogen, 19518018) by Klenow enzyme (NEB, M0210L) at 37°C for 80 min. Biotinylated DNA was ligated with 60 U T4 DNA ligase (Thermo Scientific, EL0014) in 1.2 mL final volume at room temperature for 2 h in mild agitation. .. Ligation product was reverse cross-linked by 0.5 mg/mL proteinase K (Thermo Scientific, EO0491) in 0.5% SDS, 25 mM EDTA buffer at 65°C for 4 h. The reverse cross linked sample was ethanol precipitated and purified using the Article Title: Reference genome of the bicolored carpenter ant, Camponotus vicinus Article Snippet: DNA was further cleaned with Zymo gDNA clean and concentrator kit (Zymo Research, Irvine, CA; Cat # 4033). .. To retain large DNA fragments, columns from Article Title: Manipulating the 3D organization of the largest synthetic yeast chromosome. Article Snippet: Ligation product was reverse cross-linked by 0.5 mg/mL proteinase K (Thermo Scientific, EO0492) in 25 mM EDTA buffer at 65 C for 4 hours. .. The reverse crosslinked sample was purified using the Polymerase Chain Reaction:Article Title: Radical-Mediated Nucleophilic Peptide Cross-Linking in Dynobactin Biosynthesis Article Snippet: Reaction mix: 34.5 μ L water, 2.5 μ L DMSO, 10 μ L 5× Q5 Reaction Buffer, 200 μ M (final concentration) dNTPs, 0.5 μ M Dyn_Forward and Dyn_Reverse primers ( Table S1 ), 1 μ g template DNA, and 0.02 U/ μ L Q5 DNA polymerase (New England Biolabs, Ipswich, USA). .. PCR products were purified using an agarose gel electrophoresis and the Article Title: Radical-Mediated Nucleophilic Peptide Cross-Linking in Dynobactin Biosynthesis. Article Snippet: Dynobactins are recently discovered ribosomally synthesized and post-translationally modified peptide (RiPP) antibiotics that selectively kill Gram-negative pathogens by inhibiting the β-barrel assembly machinery (Bam) located on their outer membranes.. Such activity of dynobactins derives from their unique cross-links between Trp1-Asn4 and His6-Tyr8.. In particular, the His6-Tyr8 cross-link is formed between Nτ of His6 and Cβ of Tyr8, an unprecedented type of cross-link in RiPP natural products. Agarose Gel Electrophoresis:Article Title: Radical-Mediated Nucleophilic Peptide Cross-Linking in Dynobactin Biosynthesis Article Snippet: Reaction mix: 34.5 μ L water, 2.5 μ L DMSO, 10 μ L 5× Q5 Reaction Buffer, 200 μ M (final concentration) dNTPs, 0.5 μ M Dyn_Forward and Dyn_Reverse primers ( Table S1 ), 1 μ g template DNA, and 0.02 U/ μ L Q5 DNA polymerase (New England Biolabs, Ipswich, USA). .. PCR products were purified using an agarose gel electrophoresis and the Article Title: Radical-Mediated Nucleophilic Peptide Cross-Linking in Dynobactin Biosynthesis. Article Snippet: Dynobactins are recently discovered ribosomally synthesized and post-translationally modified peptide (RiPP) antibiotics that selectively kill Gram-negative pathogens by inhibiting the β-barrel assembly machinery (Bam) located on their outer membranes.. Such activity of dynobactins derives from their unique cross-links between Trp1-Asn4 and His6-Tyr8.. In particular, the His6-Tyr8 cross-link is formed between Nτ of His6 and Cβ of Tyr8, an unprecedented type of cross-link in RiPP natural products. Ligation:Article Title: Context-dependent neocentromere activity in synthetic yeast chromosome VIII Article Snippet: DNA sticky ends were filled with biotin-14-dCTP (Invitrogen, 19518018) by Klenow enzyme (NEB, M0210L) at 37°C for 80 min. Biotinylated DNA was ligated with 60 U T4 DNA ligase (Thermo Scientific, EL0014) in 1.2 mL final volume at room temperature for 2 h in mild agitation. .. Ligation product was reverse cross-linked by 0.5 mg/mL proteinase K (Thermo Scientific, EO0491) in 0.5% SDS, 25 mM EDTA buffer at 65°C for 4 h. The reverse cross linked sample was ethanol precipitated and purified using the |